Plectin Antibody (E398P)

Images

 
Western Blot: Plectin Antibody (E398P) [NB110-57426] Jurkat cell lysate using a 1:5,000 dilution.
Immunocytochemistry/ Immunofluorescence: Plectin Antibody (E398P) [NB110-57426] - Paraffin-embedded human skeletal muscle tissue.
Immunohistochemistry: Plectin Antibody (E398P) [NB110-57426] - Immunohistochemical staining of paraffin-embedded human skeletal muscle using anti-Plectin (catalog #110-57426).

Product Details

Summary
Product Discontinued
View other related Plectin Primary Antibodies

Order Details


    • Catalog Number
      NB110-57426
    • Availability
      Product Discontinued

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Plectin Antibody (E398P) Summary

Immunogen
A recombinant fragment comprising the C-terminal 220 amino acids of plectin was used as immunogen.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PLEC
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1:40
Application Notes
This product is useful for Immunocytochemistry see literature. In Western blot this antibody detects a band at approximately 500kDa.
Theoretical MW
500 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-57426 in the following applications:

Publications
Read Publications using NB110-57426.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
49% PBS, 0.05% BSA and 50% Glycerol
Preservative
0.01% Sodium Azide
Purity
Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Plectin Antibody (E398P)

  • EBS1
  • HD1
  • Hemidesmosomal protein 1
  • LGMD2Q
  • PCNplectin 1, intermediate filament binding protein, 500kD
  • PLEC1b
  • PLEC1EBSO
  • plectin 1, intermediate filament binding protein 500kDa
  • plectin
  • plectin-1
  • PLTNepidermolysis bullosa simplex 1 (Ogna)

Background

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Plectin Antibody (NB110-57426)(3)

Reviews for Plectin Antibody (NB110-57426) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using NB110-57426:
Filter by Applications
IF
(1)
WB
(1)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Rat 12/17/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedtransfected RN22 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:10 (optimal dilution)

Details

Detection Notesn/a
Fixation Detailstransfected RN22 cells were 4% PFA in PBS for 5 min.

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
WB Rat 12/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedRN22 cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

View specific protocols for Plectin Antibody (NB110-57426): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Plectin Antibody (NB110-57426) (0)

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Secondary Antibodies

 

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Recombinant Monoclonal Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
12/17/2014
Application: IF
Species: Rat

 
Anonymous
12/17/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PLEC
Entrez
Uniprot
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