Plectin Antibody (E398P)

Product Discontinued

Plectin Antibody (E398P) Summary

• Immunogen: A recombinant fragment comprising the C-terminal 220 amino acids of plectin was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLEC
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 1:40
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for Immunocytochemistry see literature. In Western blot this antibody detects a band at approximately 500kDa.
• Theoretical MW: 500 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Plectin Antibody (E398P)

• EBS1
• HD1
• Hemidesmosomal protein 1
• LGMD2Q
• PCNplectin 1, intermediate filament binding protein, 500kD
• PLEC1b
• PLEC1EBSO
• plectin 1, intermediate filament binding protein 500kDa
• plectin
• plectin-1
• PLTNepidermolysis bullosa simplex 1 (Ogna)

Background

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plectin Antibody (NB110-57426)(3)

Publications using NB110-57426

• Takawira D, Budinger GR, Hopkinson SB et al. A dystroglycan/plectin scaffold mediates mechanical pathway bifurcation in lung epithelial cells. J Biol Chem 2011-02-01 [PMID: 21149456]
• Bhattacharya R, Gonzalez AM, Debiase PJ et al. Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength. J Cell Sci 2009-05-01 [PMID: 19366731] (ICC/IF, WB)
• Nery FC, Zeng J, NilBP et al. TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton. J Cell Sci 2008-10-01 [PMID: 18827015] (WB)

Reviews for Plectin Antibody (NB110-57426) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Rat.

reviewed by: cynthia morimoto IF Rat 12/17/2014

Summary

• Application: Immunofluorescence
• Sample Tested: transfected RN22 cells
• Species: Rat

reviewed by: cynthia morimoto WB Rat 12/17/2014

Summary

• Application: Western Blot
• Sample Tested: RN22 cells
• Species: Rat

Bioinformatics

• Gene Symbol: PLEC
• Entrez:
  • 5339(Human)
  • 18810(Mouse)
  • 64204(Rat)
• Uniprot:
  • Q15149(Human)