Plasma Kallikrein/KLKB1 Antibody (1D10)

Images

 
Western Blot: Plasma Kallikrein/KLKB1 Antibody (1D10) [NBP2-22617] - The extracts of PC-3(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLK3 antibody (1:500 ~ 1:5000). Proteins ...read more
Immunocytochemistry/ Immunofluorescence: Plasma Kallikrein/KLKB1 Antibody (1D10) [NBP2-22617] - Analysis of KLK3 in LNCap cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KLK3 antibody ...read more
Flow Cytometry: Plasma Kallikrein/KLKB1 Antibody (1D10) [NBP2-22617] - Analysis of KLK3 in LNCaP cell line, staining at 2- 5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-22617
    • Availability
      Product Discontinued

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Plasma Kallikrein/KLKB1 Antibody (1D10) Summary

Immunogen
Recombinant human KLK3(25-261aa) purified from E. coli
Specificity
Anti-human KLK3 mAb, clone 1D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KLK3 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KLKB1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
  • Flow Cytometry 2-5 ug
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Plasma Kallikrein/KLKB1 Antibody (1D10)

  • EC 3.4.21
  • EC 3.4.21.34
  • Fletcher factor
  • kallikrein B, plasma (Fletcher factor) 1
  • kininogenin
  • KLK3plasma kallikrein
  • KLKB1
  • plasma kallikrein heavy chain
  • plasma kallikrein light chain
  • Plasma Kallikrein
  • Plasma Prekallikrein
  • PPK

Background

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. PSA is now used to indicate the presence of semen in forensic serology. The semen of adult males has PSA levels far in excess of those found in other tissues; therefore, a high level of PSA found in a sample is an indicator that semen may be present. Because PSA is a biomarker that is expressed independently of spermatozoa, it remains useful in identifying semen from vasectomized and azoospermic males.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KLKB1
Entrez
Uniprot