PLA2G4A Antibody

Images

 
Western Blot: PLA2G4A Antibody [NB100-92066] - Analysis of PLA2G4A (N499) pAb at 1:500 dillutionLane1:A549 whole cell lysate(40 ug) Lane2:BV2 whole cell lysate(15 ug).
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NB100-92066] - Analyzes of PLA2G4A (N499) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: PLA2G4A Antibody [NB100-92066] - Analysis of Phospholipase at 1:500 dilution. Lane 1 - HEK293T whole cell lysate, Lane 2 - Raw264.7 whole cell lysate, Lane 3 - PC12 whole cell lysate
Immunohistochemistry-Paraffin: PLA2G4A Antibody [NB100-92066] - Analyzes in paraffin-embedded human colon carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related PLA2G4A Primary Antibodies

Order Details


    • Catalog Number
      NB100-92066
    • Availability
      Product Discontinued

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PLA2G4A Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 480-530 of Human c-PLA2.
Clonality
Polyclonal
Host
Rabbit
Gene
PLA2G4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLA2G4A Antibody

  • calcium-dependent phospholipid-binding protein
  • cPLA2
  • cPLA2-alpha
  • lysophospholipase
  • MGC126350
  • phosphatidylcholine 2-acylhydrolase
  • Phospholipase A2 group IVA
  • phospholipase A2, group IVA (cytosolic, calcium-dependent)
  • PLA2G4A
  • PLA2G4cytosolic phospholipase A2

Background

Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts; and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PLA2G4A Antibody (NB100-92066). (Showing 1 - 1 of 1 FAQ).

  1. Hi. Actually I want to purchase anti cPLA2 antibody raised in rabbit which can bind to both phorphorylated and unphosphorylated mouse cPLA2 group IV. I want to a gel shift assay with western blotting to show that my protein of interest is causing the phosphorylation of cPLA2 inside the mouse macrophages RAW cells.Can you please tell me which antibody would be suitable for the purpose. Thanks a lot.
    • Here are our antibodies raised in rabbit that have been validated to detect cPLA2 in mouse samples in a western blot. We have not specifically confirmed the ability of these antibodies to detect both the phosphorylated and unphosphorylated forms of the protein, and I cannot guarantee that they will clearly differentiate between the two.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLA2G4A
Entrez
Uniprot