Recombinant Human PKLR Protein Summary
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 47-574 of Human PKLR
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
|Details of Functionality
Specific activity: > 80 units/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.
| Protein/Peptide Type
Biologically Active Protein
>90%, by SDS-PAGE
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
>90%, by SDS-PAGE
Alternate Names for Recombinant Human PKLR Protein
- EC 22.214.171.124
- Pyruvate Kinase 1
- pyruvate kinase isozyme R/L
- pyruvate kinase isozymes R/L
- pyruvate kinase type L
- pyruvate kinase, liver and blood cell
- pyruvate kinase, liver and RBC
- Red cell/liver pyruvate kinase
- R-type/L-type pyruvate kinase
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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