Recombinant Human PKLR Protein

Images

 
SDS-Page: Recombinant Human PKLR Protein [NBP1-48392] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human PKLR Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 47-574 of Human PKLR

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS

Details of Functionality
Specific activity: > 80 units/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
PKLR
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
59.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PKLR Protein

  • EC 2.7.1.40
  • PK1
  • PK1PKRL
  • PKL
  • PKLR
  • PKR
  • Pyruvate Kinase 1
  • pyruvate kinase isozyme R/L
  • pyruvate kinase isozymes R/L
  • pyruvate kinase type L
  • pyruvate kinase, liver and blood cell
  • pyruvate kinase, liver and RBC
  • Red cell/liver pyruvate kinase
  • RPK
  • R-type/L-type pyruvate kinase

Background

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-351
Species: Hu, Ha, Pm, Mu(-), Rt(-)
Applications: WB, ELISA, IHC, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP2-02669
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-47859
Species: Hu, Mu, Mk
Applications: WB, ICC/IF
NBP1-48308
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-78017
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
MAB1980
Species: Hu
Applications: WB, Simple Western, IP, ICC
NBP2-43853
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, ICC/IF, IHC-Fr, IHC-P
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NBP2-48704
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57614
Species: Hu
Applications: ICC/IF
NBP2-27109
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western, IHC, IHC-P
AF4715
Species: Mu
Applications: WB
NBP1-48392
Species: Hu
Applications: Func, PAGE

Publications for PKLR Protein (NBP1-48392) (0)

There are no publications for PKLR Protein (NBP1-48392).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PKLR Protein (NBP1-48392) (0)

There are no reviews for PKLR Protein (NBP1-48392). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for PKLR Protein (NBP1-48392) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PKLR Products

Bioinformatics Tool for PKLR Protein (NBP1-48392)

Discover related pathways, diseases and genes to PKLR Protein (NBP1-48392). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PKLR Protein (NBP1-48392)

Discover more about diseases related to PKLR Protein (NBP1-48392).
 

Pathways for PKLR Protein (NBP1-48392)

View related products by pathway.

PTMs for PKLR Protein (NBP1-48392)

Learn more about PTMs related to PKLR Protein (NBP1-48392).
 

Research Areas for PKLR Protein (NBP1-48392)

Find related products by research area.

Blogs on PKLR

There are no specific blogs for PKLR, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PKLR Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol PKLR
Entrez