PKLR Recombinant Protein Summary
A recombinant protein with a His tag and corresponding to amino acids 47 - 574 of PKLR.
Source: E. coli
Amino Acid Sequence: MGSSHHHHHHSSGLVPRGSHMLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
|Details of Functionality
Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 59.2 kDa (549aa), confirmed by MALDI-TOF
1. Prepare a 1.45 ml reaction mixture into a suitable container.
- Reaction mixture: 100mM Tris-HCl pH 7.5, 7.6 mM ADP, 15 mM MgCl2, 74 mM KCl, 0.2 mM Beta-NADH 5.2 mM PEP, 0.025 units Recombinant LDHA protein (ATGen Cat. No, ATGP0488)
2. Add 50ul of recombinant PKLR protein solution with various concentrations (0.5ug, 1ug)
3. Read the decrease in A340nm in kinetic mode for 10 minutes.
Alternate Names for PKLR Recombinant Protein
- EC 220.127.116.11
- Pyruvate Kinase 1
- pyruvate kinase isozyme R/L
- pyruvate kinase isozymes R/L
- pyruvate kinase type L
- pyruvate kinase, liver and blood cell
- pyruvate kinase, liver and RBC
- Red cell/liver pyruvate kinase
- R-type/L-type pyruvate kinase
PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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