Recombinant Human PKC zeta Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
0.1 mg/ml

Order Details

Recombinant Human PKC zeta Protein Summary

Description
Recombinant protein for Human PKC zeta

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(Q05513)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Recombinant Protein
Gene
PRKCZ
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: 93 nmol phosphate incorporated into CREBtide per minute per mg protein at 300C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PKC zeta Protein

  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-zeta
  • PKC zeta
  • PKC2
  • PKC2PKC-ZETA
  • PRKCZ
  • protein kinase C zeta type
  • protein kinase C, zeta

Background

PKC? (PKC zeta) is an atypical isoform of the PKC family. PKC? is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Treatment of cells with phorbol ester which activates PKCa, gamma, d, and e isoforms in NIH3T3 cells significantly reduced proliferation of cells. Overexpression of PKC? and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKC? also potentiated phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. The effects of PKC? overexpression on proliferation and MAP kinase activation are proportional to the levels of PKC? expression. PKC? as an upstream modulator of p70S6K, an important regulator of cell proliferation (2). Kinase-inactive PKC? mutant antagonized activation of p70S6K by epidermal growth factor, PDK-1, and activated Cdc42 and PI3-K. Overexpression of a constitutively active PKC? mutant (myristoylated PKC? [myr-PKCzeta]) only modestly activated p70S6K but this mutant cooperated with PDK-1 for the activation of p70S6K. PDK-1-induced activation of a C-terminal truncation mutant of p70S6K was also enhanced by myr-PKC?. p70S6K can associate with both PDK-1 and PKCz in vivo in a growth factor-independent manner, while PDK-1 and PKC? can also associate with each other, suggesting the existence of a multimeric PI3-K signalling complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PRKCZ
Entrez