PKC theta [p Ser676] Antibody

Images

 
Western Blot: PKC theta [p Ser676] Antibody [NBP1-51414] - Analysis of p-PKC Theta pAb in extracts from Jurkat cells.
Immunohistochemistry-Paraffin: PKC theta [p Ser676] Antibody [NBP1-51414] - Analysis of p-PKC theta pAb in paraffin-embedded human breast carcinoma cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51414
    • Availability
      Product Discontinued

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PKC theta [p Ser676] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human PKC theta around the phosphorylation site of Serine 676.
Modification
p Ser676
Specificity
p-PKC theta (S676) pAb detects endogenous levels of PKC theta protein only when phosphorylated at S676.
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCQ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-PKC Theta (S676) pAb in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of p-PKC Theta (S676) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC theta [p Ser676] Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC126514
  • MGC141919
  • nPKC-theta
  • PKC theta
  • PRKCQ
  • PRKCT
  • protein kinase C theta type
  • protein kinase C, theta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in a



variety of cellular functions including cell growth and differentiation, gene



expression, hormone secretion and membrane function. PKCs were originally



identified as serine/threonine protein kinases whose activity was dependent



on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting



phorbol esters bind to and activate PKC. PKCs can be subdivided into many



different isoforms (a, BI, BII, y, delta, sig, Z, n, theta, I, lamda, u and v). Patterns of expression



for each PKC isoform differ among tissues and PKC family members



exhibit clear differences in their cofactor dependencies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PRKCQ
Entrez