Recombinant Human PKC mu Protein

Images

 
There are currently no images for PKC mu Biologically Active Protein (NBP1-99477).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, HPLC
Concentration
0.1 mg/ml

Order Details

Recombinant Human PKC mu Protein Summary

Description
Recombinant protein for Human PKC mu

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(Q15139)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Biologically Active Protein
Gene
PRKD1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: 680 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PKC mu Protein

  • EC 2.7.11
  • nPKC-D1
  • nPKC-mu
  • PKCM
  • PKC-MU
  • PKD1
  • PKDEC 2.7.11.13
  • PRKCMPKC-mu
  • PRKD1
  • Protein kinase C mu type
  • protein kinase C, mu
  • Protein kinase D
  • protein kinase D1
  • serine/threonine-protein kinase D1

Background

Protein kinase Cmu (PKCmu) is a novel member of the protein kinase C (PKC) family that differs from the other isoenzymes in structural and enzymatic properties. It is characterized by the presence of a pleckstrin homology (PH) domain and an amino-terminal hydrophobic region and has substrate specificity distinct from other PKC isoforms. PKCmu is a ubiquitous PKC isotype with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKCmu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCmu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). PKCmu was also shown to associate with the B cell receptor (BCR) complex and its activity is up-regulated after cross-linking the BCR and CD19 on B cells (3). PKC mu co-precipitates with Syk and phospholipase C-gamma 1/2 (PLC gamma 1/2) and in vitro phosphorylation of fusion proteins showed that both Syk and PLC gamma 1 are potential substrates of PKC mu in vivo. In addition, specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays (4). 14-3-3tau is not a substrate of PKCmu and strongly down-regulates PKCmu kinase activity in vitro. In response to various stimuli, PKC mu activates the mitogen-activated protein kinase (p42/ERK1 MAPK cascade) but does not affect the related c-jun N-terminal kinase or p38 MAPK (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-80944
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-02984
Species: Hu, Rt, Ca, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
BC110-60480
Species: Hu, Mu
Applications: ICC/IF, PEP-ELISA
NBP1-87948
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-22152
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-27561
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-14868
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB40401
Species: Hu, Mu
Applications: WB, IHC, ICC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
H00005122-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for PKC mu Biologically Active Protein (NBP1-99477) (0)

There are no publications for PKC mu Biologically Active Protein (NBP1-99477).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PKC mu Biologically Active Protein (NBP1-99477) (0)

There are no reviews for PKC mu Biologically Active Protein (NBP1-99477). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for PKC mu Biologically Active Protein (NBP1-99477) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PKC mu Products

Bioinformatics Tool for PKC mu Biologically Active Protein (NBP1-99477)

Discover related pathways, diseases and genes to PKC mu Biologically Active Protein (NBP1-99477). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PKC mu Biologically Active Protein (NBP1-99477)

Discover more about diseases related to PKC mu Biologically Active Protein (NBP1-99477).
 

Pathways for PKC mu Biologically Active Protein (NBP1-99477)

View related products by pathway.

PTMs for PKC mu Biologically Active Protein (NBP1-99477)

Learn more about PTMs related to PKC mu Biologically Active Protein (NBP1-99477).
 

Research Areas for PKC mu Biologically Active Protein (NBP1-99477)

Find related products by research area.

Blogs on PKC mu

There are no specific blogs for PKC mu, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PKC mu Antibody
NBP1-87789

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PKC mu Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol PRKD1
Entrez