PKC iota Antibody

Images

 
Western Blot: PKC iota Antibody [NBP1-32413] - (30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: PKC iota Antibody [NBP1-32413] - Paraformaldehyde-fixed HeLa, using PKC iota antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin ...read more
Immunohistochemistry-Paraffin: PKC iota Antibody [NBP1-32413] - Paraffin-embedded Gastric T, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PKC iota Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PKC iota. The exact sequence is proprietary.
Localization
Cytoplasm, Membrane, Endosome, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (91%) and Xenopus tropicalis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC iota Antibody

  • aPKC-lambda/iota
  • Atypical protein kinase C-lambda/iota
  • DXS1179E
  • EC 2.7.11
  • EC 2.7.11.13
  • MGC26534
  • nPKC-iota
  • PKCI
  • PRKC-lambda/iota
  • protein kinase C iota type
  • protein kinase C, iota

Background

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKC iota Antibody (NBP1-32413) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKCI