PKC iota Antibody


Western Blot: PKC iota Antibody [NBP1-32413] - (30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: PKC iota Antibody [NBP1-32413] - Paraformaldehyde-fixed HeLa, using PKC iota antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin more
Immunohistochemistry-Paraffin: PKC iota Antibody [NBP1-32413] - Paraffin-embedded Gastric T, using antibody at 1:100 dilution.

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PKC iota Antibody Summary

Recombinant protein encompassing a sequence within the center region of human PKC iota. The exact sequence is proprietary.
Cytoplasm, Membrane, Endosome, Nucleus
Immunogen affinity purified
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  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Positive Control
PKC iota Lysate (NBP2-64715)

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (91%) and Xenopus tropicalis (91%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
0.01% Thimerosal
Immunogen affinity purified

Alternate Names for PKC iota Antibody

  • aPKC-lambda/iota
  • Atypical protein kinase C-lambda/iota
  • DXS1179E
  • EC 2.7.11
  • EC
  • MGC26534
  • nPKC-iota
  • PKCI
  • PRKC-lambda/iota
  • protein kinase C iota type
  • protein kinase C, iota


This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol PRKCI