PKC epsilon Antibody (3A12)

Images

 
Western Blot: PKC epsilon Antibody (3A12) [NBP2-00475] Analysis of extracts (35ug) from 9 different cell lines by using anti-PKC epsilon monoclonal antibody.
Immunohistochemistry-Paraffin: PKC epsilon Antibody (3A12) [NBP2-00475] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKC epsilon mouse monoclonal antibody.
Flow Cytometry: PKC epsilon Antibody (3A12) [NBP2-00475] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKC epsilon antibody, and ...read more
Western Blot: PKC epsilon Antibody (3A12) [NBP2-00475] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Flow Cytometry: PKC epsilon Antibody (3A12) [NBP2-00475] - Analysis of Hela cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PKC epsilon Antibody (3A12) [NBP2-00475] - Analysis of Jurkat cells, using anti-PKC epsilon antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00475
    • Availability
      Product Discontinued

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PKC epsilon Antibody (3A12) Summary

Immunogen
Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKCE
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PKC epsilon Antibody (3A12)

  • EC 2.7.11
  • EC 2.7.11.13
  • MGC125656
  • nPKC-epsilon
  • PKC epsilon
  • PKCE
  • PKCEMGC125657
  • PRKCE
  • protein kinase C epsilon type
  • protein kinase C, epsilon

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRKCE
Entrez