PKC delta [p Thr507] Antibody

Images

 
Western Blot: PKC delta [p Thr507] Antibody [NBP1-51405] - Lane 1: HeLa Lane 2: RAW264.7 Lane 3: PC12 Lane 4: A549 all treated with H2O2
Immunohistochemistry-Paraffin: PKC delta [p Thr507] Antibody [NBP1-51405] - Paraffin-embedded human breast carcinoma tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51405
    • Availability
      Product Discontinued

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PKC delta [p Thr507] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human PKC alpha around the phosphorylation site of Threonine 505.
Modification
p Thr507
Specificity
p-PKC delta (T507) pAb detects endogenous levels of PKC delta protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PRKCD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-PKC Delta (T507) pAb in extracts from NIH-3T3 cells treated with UV.
Immunohistochemistry (IHC) analyzes of p-PKC Delta (T507) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKC delta [p Thr507] Antibody

  • EC 2.7.11
  • EC 2.7.11.13
  • MAY1
  • MGC49908
  • nPKC-delta
  • PKC delta
  • PKCD
  • PRKCD
  • protein kinase C delta type
  • protein kinase C delta VIII
  • protein kinase C, delta

Background

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (alpha, beta-I, beta-II, gamma, delta, epsilon, zeta, nu, theta, L, lambda, mu and V). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC delta and episol are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKCD
Entrez
Uniprot