PKA R2 Antibody (1B4)

Images

 
Western Blot: PKA R2 Antibody (1B4) [NBP2-02517] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA R2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PKA R2 Antibody (1B4) [NBP2-02517] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA R2.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded prostate tissue using anti-PKA R2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PKA R2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PKA R2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PKA R2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded Kidney tissue using anti-PKA R2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PKA R2 Antibody (1B4) [NBP2-02517] - Staining of paraffin-embedded pancreas tissue using anti-PKA R2 mouse monoclonal antibody.

Product Details

Summary
Product Discontinued
View other related PKA R2 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-02517
    • Availability
      Product Discontinued

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PKA R2 Antibody (1B4) Summary

Immunogen
Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAR2A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PKA R2 Antibody (1B4)

  • protein kinase, cAMP-dependent, regulatory, type II, alpha
  • RII-alpha subunit

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRKAR2A
Entrez