PKA C alpha/beta Antibody

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Western Blot: cAMP Protein Kinase Catalytic subunit alpha/beta Antibody [NBP2-13046] - Extracts from Raw264.7 cells.

Product Details

Summary
Product Discontinued
View other related PKA C alpha/beta Primary Antibodies

Order Details


    • Catalog Number
      NBP2-13046
    • Availability
      Product Discontinued

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PKA C alpha/beta Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human PKAalpha around the phosphorylation site of Threonine 197.
Specificity
Detects both PKA C-alpha(Gene ID: 5566, UniProt: P17612 ) and PKA C-beta(Gene ID: 5567, UniProt: P22694 ) proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-PKAalpha /Beta cat (T197) pAb in extracts from Raw264.7 cells.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKA C alpha/beta Antibody

  • PKA C alpha/beta
  • PRKACA/B

Background

PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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