PKA 2 beta Antibody

Images

 
Western Blot: PKA 2 beta Antibody [NBP1-00983] - Western blot (WB) analysis of PKA 2 beta reg (T109) antibody in extracts from Jurkat cells
Immunohistochemistry: PKA 2 beta Antibody [NBP1-00983] - Immunohistochemistry (IHC) analyzes of PKA 2 beta reg (T109) antibody in paraffin-embedded human cervix carcinoma tissue

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00983
    • Availability
      Product Discontinued

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PKA 2 beta Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 80-130 of Human PKA IIbeta reg.
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAR2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PKA 2 beta reg (T109) pAb in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of PKA 2 beta reg (T109) pAb in paraffin-embedded human cervix carcinoma tissue.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PKA 2 beta Antibody

  • cAMP-dependent protein kinase type II-beta regulatory chain
  • cAMP-dependent protein kinase type II-beta regulatory subunit
  • H_RG363E19.2
  • PRKAR2
  • protein kinase, cAMP-dependent, regulatory, type II, beta
  • RII-BETA
  • WUGSC:H_RG363E19.2

Background

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, that each represent specific gene products. C-alpha and C-beta are closely related (93% amino acid sequence similarity), whereas C-gamma displays 83% and 79% similarity to C-alpha and C-beta, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol PRKAR2B
Entrez
Uniprot