PiColorLock Gold Colorimetric Assay Kit

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PiColorlock Gold Colorimetric Assay Kit [303-0030] - Universal assay for inorganic phosphate. Phosphorylated substrate (X-P) is acted on by an enzyme releasing X and inorganic phosphate. The reaction is halted by the ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - Most detection reagents form very unstable dye complexes and samples need to be read promptly. In cuvette assays there can be a total loss of signal if a precipitate ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - For the purposes of comparison, data for PiColorlock ALS reagent (no longer available) is also shown, as it provides similar absorbance values to competitor products, ...read more
PiColorlock Gold Colorimetric Assay Kit [303-0030] - Shows the effect of incubating ATP (without any added enzyme) with three commercial dye-based detection reagents.

Product Details

Summary
Kit Type
Colorimetric Assay Kit

Order Details

PiColorLock Gold Colorimetric Assay Kit Summary

Description
PiColorLock phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.

The unique formulation of PiColorLock affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.

FeaturesBenefits
Colorimetric assaySimple, scalable and non-radioactive
Compatible with almost any assay bufferFlexibility over a wide range of existing assays
Stable reagent formulationLong shelf life
Unique accelerator and stabilizerSpeeds up color development
PiColorLock-Pi complex is very stableNo precipitation, results can be measured over several hours - ideal for HTS
Wide linear rangeNo inhibition of color development by high concentrations of protein
Kit Type
Colorimetric Assay Kit

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
303-0030 in the following application:

Publications
Read Publications using 303-0030.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Colorlock 635/1560 assays reagent is supplied with a phosphate standard solution.

Notes

PiColorlock® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for PiColorLock Gold Kit (303-0030)(60)


Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using 303-0030 Applications Species
Choi K, Batke S, Szakal B et al. Concerted and differential actions of two enzymatic domains underlie Rad5 contributions to DNA damage tolerance. Nucleic Acids Res 2015 [PMID: 25690888]
Arnold LH, Kunzelmann S, Webb MR, Taylor IA. A continuous enzyme-coupled assay for triphosphohydrolase activity of HIV-1 restriction factor SAMHD1. Antimicrob Agents Chemother. 2014 [PMID: 25331707]
Humnabadkar V, Madhavapeddi P, Basavarajappa H et al. Assays, Surrogates, and Alternative Technologies for a TB Lead Identification Program Targeting DNA Gyrase ATPase. J Biomol Screen 2014 [PMID: 25300873]
Menard R, Schoenhofen IC, Tao L et al. Small-molecule inhibitors of the pseudaminic acid biosynthetic pathway: targeting motility as a key bacterial virulence factor. Antimicrob Agents Chemother. 2014 [PMID: 25267679]
Sarraf NS, Shi R, McDonald L et al. Structure of CbpA J-Domain Bound to the Regulatory Protein CbpM Explains Its Specificity and Suggests Evolutionary Link between CbpM and Transcriptional Regulators. PLoS One 2014 [PMID: 24945826]
Zheng M, Chiang YL, Lee HL et al. Self-Assembly of MinE on the Membrane Underlies Formation of the MinE-Ring to Sustain Function of the E. coli Min System. J Biol Chem 2014 [PMID: 24914211]
Ellis LC, Grant MH, Hawksworth GM, Weaver RJ. Quantification of biliary excretion and sinusoidal excretion of 5(6)-carboxy-2',7'-dichlorofluorescein (CDF) in cultured hepatocytes isolated from Sprague Dawley, Wistar and Mrp2-deficient Wistar (TR(-)) rats. Toxicol In Vitro 2014 [PMID: 24907646]
Selwa E, Davi M, Chenal A et al. Allosteric Activation of Bordetella pertussis Adenylyl Cyclase by Calmodulin: Molecular Dynamics and Mutagenesis Studies. J Biol Chem 2014 [PMID: 24907274]
Gundner AL, Hahn I, Sendscheid O et al. The PIKE Homolog Centaurin gamma Regulates Developmental Timing in Drosophila. PLoS One 2014 [PMID: 24845618]
Yadav MC, Huesa C, Narisawa S et al. Ablation of Osteopontin Improves the Skeletal Phenotype of Phospho1-/- Mice. J Bone Miner Res 2014 [PMID: 24825455]
Show All 60 Publications.

Review for PiColorLock Gold Kit (303-0030) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: ELISA.

Reviews using 303-0030:
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ELISA PiColorLock Gold 303-0030
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reviewed by:
Hilary Frase
ELISA Other 06/08/2012
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Summary

ApplicationELISA
Sample Testedpurified recombinant enzyme
SpeciesOther
LotGIBS161
CommentsI used this product to measure Pi release by a recombinant enzyme. I was able to do this in a 96-well plate format and even decreased the reagent used to 1/2 when I used 1/2 well plates. I was extremely pleased with the linearity of the reagent to Pi concentration and the long stability of the signal!

Blocking

Blocking Detailsnone

Primary Anitbody

Dilution Ration/a

Details

Detection NotesPi ColorLock Gold
ELISA Plate Detailsuncoated
Wash Descriptionnone

Comments

CommentsI used this product to measure Pi release by a recombinant enzyme. I was able to do this in a 96-well plate format and even decreased the reagent used to 1/2 when I used 1/2 well plates. I was extremely pleased with the linearity of the reagent to Pi concentration and the long stability of the signal!

FAQs for PiColorLock Gold Kit (303-0030). (Showing 1 - 4 of 4 FAQs).

  1. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.

Additional PiColorLock Gold Products

PiColorLock Gold 303-0030

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Hilary Frase
06/08/2012
Application: ELISA
Species: Other