PI 3-Kinase p85 alpha Antibody

Images

 
Western Blot: PI 3-Kinase p85 alpha Antibody [NBP1-51261] - Analysis of PI3K p85 alpha (Q498) pAb at 1:500 dilution Lane1:NIH-3T3 whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug)
Immunohistochemistry-Paraffin: PI 3 Kinase p85 alpha Antibody [NBP1-51261] Human lung carcinoma tissue.
Western Blot: PI 3 Kinase p85 alpha Antibody [NBP1-51261] - Extracts from HELA cells.

Product Details

Summary
Product Discontinued
View other related PI 3-Kinase p85 alpha Primary Antibodies

Order Details


    • Catalog Number
      NBP1-51261
    • Availability
      Product Discontinued

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PI 3-Kinase p85 alpha Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 470-510 of Human PI3K p85alpha.
Specificity
PI 3-kinase p85alpha (Q498) pAb detects endogenous levels of PI 3-kinase p85alpha protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3R1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of PI 3-kinase p85alpha (Q498) pAb in extracts from HELA cells.
Immunohistochemistry (IHC) analyzes of PI 3-kinase p85alpha (Q498) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PI 3-Kinase p85 alpha Antibody

  • GRB1
  • GRB1PI3-kinase subunit p85-alpha
  • p85
  • p85-ALPHA
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • phosphatidylinositol 3-kinase regulatory subunit alpha
  • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
  • phosphatidylinositol 3-kinase, regulatory, 1
  • phosphatidylinositol 3-kinase-associated p-85 alpha
  • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
  • PI 3Kinase p85 alpha
  • PI 3-Kinase p85 alpha
  • PI3K regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PIK3R1
  • PtdIns-3-kinase regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIK3R1
Entrez