phycoerythrin Antibody (PE001)

Images

 
Flow Cytometry: phycoerythrin Antibody (PE001) [NB100-78114] - Analysis using the Biotin conjugate of NB100-78114. Stained of RM4-5 PE plus biotinylated PE001 then detected with Sav-FITC.
Flow Cytometry: phycoerythrin Antibody (PE001) [NB100-78114] - C57BL/6 splenocytes stained with RM4-5 PE and purified PE001, then detected with anti-mouse IgG1 FITC.
Flow Cytometry: phycoerythrin Antibody (PE001) [NB100-78114] - Analysis using the Biotin conjugate of NB100-78114. Staining of C57BL/6 thymocytes with RM4-5 PE.

Product Details

Summary
Product Discontinued
View other related phycoerythrin Primary Antibodies

Order Details


    • Catalog Number
      NB100-78114
    • Availability
      Product Discontinued

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phycoerythrin Antibody (PE001) Summary

Immunogen
Phycoerythrin
Specificity
phycoerythrin (PE001)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Use at concentrations: 0.5 mug/test. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

All Species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for phycoerythrin Antibody (PE001)

  • PE
  • R-PE

Background

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cy5, PE/Cy5.5 and PE/Cy7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional phycoerythrin Products

phycoerythrin NB100-78114

Blogs on phycoerythrin

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Calculators

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