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PHPT1 Antibody

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Western Blot: PHPT1 Antibody [NBP2-78799] - Samples: Whole cell lysate (50 ug) from GaMG, RKO, LNCaP, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHPT1 antibody NBP2-78799 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: PHPT1 Antibody [NBP2-78799] - Samples: Whole cell lysate (50 ug) from GaMG, RKO, LNCaP, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHPT1 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm-OrSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PHPT1 Antibody Summary

Immunogen
between 75 and 125
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHPT1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
TBS containing 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for PHPT1 Antibody

  • bA216L13.10
  • DKFZp564M173
  • EC 3.1.3,14 kDa phosphohistidine phosphatase
  • EC 3.1.3.-
  • HSPC141
  • phosphohistidine phosphatase 11700008C22Rik
  • phosphohistidine phosphatase 14kDa
  • PHP14CGI-202
  • Protein janus-A homolog
  • RP11-216L13.10
  • sex-regulated protein janus-a

Background

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(3 Publications)
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Applications: WB, ICC
ASAH1 Antibody
NBP1-89296
Western Blot: ASAH1 Antibody [NBP1-89296] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-ASAH1 antibody. Corresponding ASAH1 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP1-89296] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
(6 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
KCNN4 Antibody
NBP2-33694
Immunohistochemistry-Paraffin: KCNN4 Antibody [NBP2-33694] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: KCNN4 Antibody [NBP2-33694] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: KCNN4 Antibody [NBP2-33694] - Staining of human colon shows strong cytoplasmic/ membranous positivity in smooth muscle cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Recombinant Human
HIST1H4I GST (N-Term) ...
H00008294-P01
SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Histone H4 [ac
Lys5] Antibody
NB21-2024
Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.
Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
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Species: Hu, Mu, Rt, Po, Ma
Applications: WB, ChIP, DB, ICC/IF
ATP Citrate Lyase
Antibody
NBP1-90266
Western Blot: ATP Citrate Lyase Antibody [NBP1-90266] - Analysis using Anti-ACLY antibody NBP1-90266 (A) shows similar pattern to independent antibody NBP1-90268 (B).
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90266] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Western Blot: ATP Citrate Lyase Antibody [NBP1-90266] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACLY antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, KD
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Recombinant Human
H4/e GST (N-Term) Pro ...
H00008363-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Recombinant Human
H4/d GST (N-Term) Pro ...
H00008362-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
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H4/h Antibody (6D4)
H00008365-M01
Immunocytochemistry/Immunofluorescence: H4/h Antibody (6D4) [H00008365-M01] - Analysis of monoclonal antibody to HIST1H4H on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: H4/h Antibody (6D4) [H00008365-M01] - Detection limit for recombinant GST tagged HIST1H4H is 0.1 ng/ml as a capture antibody.
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA
H4/j
Overexpression Lysate
NBL1-11567
Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.
Species: Hu
H4/n Antibody
(3E7H2)
NBP2-52486
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Glutathione
S-Transferase pi 1/GS ...
H00002950-M01
Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of GSTP1 expression in transfected 293T cell line by GSTP1 monoclonal antibody (M01), clone 2G6-F6.Lane 1: GSTP1 transfected lysate (Predicted MW: 23.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of monoclonal antibody to GSTP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: Glutathione S-Transferase pi 1/GSTP1 Antibody (2G6-F6) [H00002950-M01] - Analysis of GSTP1 transfected lysate using anti-GSTP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GSTP1 MaxPab rabbit polyclonal antibody.
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H00004830-M02
Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
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Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Serpin A1/alpha
1-Antitrypsin Antibod
NBP1-90309
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis using Anti-SERPINA1 antibody NBP1-90309 (A) shows similar pattern to independent antibody NBP1-90308 (B).
Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human placenta shows strong positivity in plasma in blood vessels.
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SUCLG2 Antibody
NBP1-32521
Western Blot: SUCLG2 Antibody [NBP1-32521] - Wild-type (WT) and SUCLG2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SUCLG2 Antibody [NBP1-32521] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLG2 protein stained by SUCLG2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SUCLG2 Antibody [NBP1-32521] - Paraffin-embedded hepatoma. SUCLG2 antibody dilution: 1:500
(4 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Thromboxane
synthase Antibody (OT
NBP2-02710
Western Blot: Thromboxane synthase Antibody (2C1) [NBP2-02710] Analysis of extracts (35ug) from 9 different cell lines by using anti-Thromboxane synthase monoclonal antibody.
Immunocytochemistry/Immunofluorescence: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Thromboxane synthase.
Immunohistochemistry-Paraffin: Thromboxane synthase Antibody (2C1) [NBP2-02710] - Staining of paraffin-embedded Human liver tissue using anti-Thromboxane synthase mouse monoclonal antibody.
(1 Publication)
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol PHPT1
Uniprot