Phosphoserine/Phosphothreonine/Phosphotyrosine Antibody (SPM101)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
SPM101
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Phosphoserine/Phosphothreonine/Phosphotyrosine Antibody (SPM101) Summary

Immunogen
Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers.
Localization
Cell Membrane and Cytoplasmic
Specificity
This recognizes serine, threonine, and tyrosine phosphorylated proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MCF-7 Lysate (NB820-59465)
Publications
Read Publication using NB600-558.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Background

Protein phosphorylation provides a signalling system that can be thought of as a kind of protein on/off switch for many cellular signalling pathways. Phosphorylation is observed on serine, threonine, tyrosine and histidine residues. Cellular networks underlying phosphorylation can be very complex and often occurs on multiple distinct sites on a given protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphoserine/Phosphothreonine/Phosphotyrosine Antibody (NB600-558)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Phosphoserine/Phosphothreonine/Phosphotyrosine Antibody (NB600-558). (Showing 1 - 2 of 2 FAQ).

  1. What is the band size I should expect if using this antibody for western blot?
    • NB600-558 will detect any protein that is phosphorylated at serine residues. Therefore, the band size and number of bands will vary greatly depending on the phosphorylation levels of your samples and the types of proteins expressed.
  2. What is the concentration of this antibody?
    • The concentration of this product is 0.2mg/ml.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Phosphoserine/Phosphothreonine/Phosphotyrosine Products

Phosphoserine/Phosphothreonine/Phosphotyrosine NB600-558

Research Areas for Phosphoserine/Phosphothreonine/Phosphotyrosine Antibody (NB600-558)

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