A lysate from Jurkat human acute T cell leukemia cells was immunoprecipitated with the Phospho-SHP-2 (Y542) Capture Antibody. Unbound material was removed by washing. The bound material was solubilized in SDS-PAGE ...read more
NIH-3T3 mouse embryonic fibroblast cells were treated with 50 ng/mL of recombinant human PDGF-BB (Catalog # 220-BB) for 20 minutes. The lysates were either quantified with this DuoSet® IC ELISA or immunoblotted ...read more
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Alternate Names for Phospho-SHP-2 (Y542) DuoSet IC ELISA, 2 Plate
protein tyrosine phosphatase, non-receptor type 11
protein tyrosine phosphatase-2
Protein-tyrosine phosphatase 1D
Protein-tyrosine phosphatase 2C
SH-PTP2Noonan syndrome 1
tyrosine-protein phosphatase non-receptor type 11
The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.