PHLPP Antibody

Images

 
Western Blot: PHLPP Antibody [NB100-1811] - Detection of Human and Mouse PHLPP on HeLa whole cell lysate using NB100-1811. PHLPP was also immunoprecipitated using rabbit anti-PHLPP antibody NB100-1810.

Product Details

Summary
Product Discontinued
View other related PHLPP Primary Antibodies

Order Details


    • Catalog Number
      NB100-1811
    • Availability
      Product Discontinued

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PHLPP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1205) of human PH Domain and Leucine Rich Repeat Protein Phosphatase using the numbering given in entry NP_919431.1 (GeneID 23239).
Specificity
PHLPP antibody has been reported to cross reacts with beta-catenin, which may display 90kDa band. We would not recommend using this specific antibody for assays where signals may not be notable between PHLPP1 and Beta-Catenin.
Clonality
Polyclonal
Host
Rabbit
Gene
PHLPP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
Western Blot: Beta catenin band in Western blot is relatively less intense than PHLPP band. We do not recommend this antibody in Immunohistochemistry, Immunocytochemistry and Immunofluorescence due to its cross reactivity to Beta Catenin. Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-Paraffin reported in scientific literature (PMID 25820252)
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PHLPP Lysate (NBL1-14370)
Publications
Read Publications using
NB100-1811 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHLPP Antibody

  • EC 3.1.3.16
  • hSCOP
  • KIAA0606
  • MGC161555
  • PH domain and leucine rich repeat protein phosphatase 1
  • PH domain and leucine rich repeat protein phosphatase
  • PH domain leucine-rich repeat-containing protein phosphatase 1
  • PHLPP
  • pleckstrin homology domain containing, family E (with leucine rich repeats)member 1
  • Pleckstrin homology domain-containing family E member 1
  • PLEKHE1
  • SCN circadian oscillatory protein
  • SCOPPH domain-containing family E member 1
  • Suprachiasmatic nucleus circadian oscillatory protein

Background

PHLPP (PH domain leucine-rich repeat-containing protein phosphatase) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPP has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPP functions similarly to its relative PHLPPL, also known as PHLPPL2. Recent studies show that PHLPP and PHLPPL selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPP include pleckstrin homology domain-containing family E protein 1, suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PLEKHE1, and SCOP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PHLPP Antibody (NB100-1811)(5)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PHLPP1
Entrez
Uniprot