PHLPP Antibody

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Immunoprecipitation: PHLPP Antibody [NB100-1810] - Detection of Human PHLPP on HeLa whole cell lysate using NB100-1810 and NB100-1811. Immunoprecipitated PHLPP blotted with NB100-1810.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-1810
    • Availability
      Product Discontinued

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PHLPP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1050 and 1100 of human PH Domain and Leucine Rich Repeat Protein Phosphatase using the numbering given in entry NP_919431.1 (GeneID 23239).
Clonality
Polyclonal
Host
Rabbit
Gene
PHLPP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1-4 ug/mg of lysate
Positive Control
PHLPP Lysate (NBL1-14370)
Publications
Read Publications using NB100-1810.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHLPP Antibody

  • EC 3.1.3.16
  • hSCOP
  • KIAA0606
  • MGC161555
  • PH domain and leucine rich repeat protein phosphatase 1
  • PH domain and leucine rich repeat protein phosphatase
  • PH domain leucine-rich repeat-containing protein phosphatase 1
  • PHLPP
  • pleckstrin homology domain containing, family E (with leucine rich repeats)member 1
  • Pleckstrin homology domain-containing family E member 1
  • PLEKHE1
  • SCN circadian oscillatory protein
  • SCOPPH domain-containing family E member 1
  • Suprachiasmatic nucleus circadian oscillatory protein

Background

PHLPP (PH domain leucine-rich repeat-containing protein phosphatase) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPP has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPP functions similarly to its relative PHLPPL, also known as PHLPPL2. Recent studies show that PHLPP and PHLPPL selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPP include pleckstrin homology domain-containing family E protein 1, suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PLEKHE1, and SCOP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PHLPP Antibody (NB100-1810)(7)


Showing Publications 1 - 7 of 7.
Publications using NB100-1810 Applications Species
Warfel NA, Niederst M, Stevens MW et al. Mislocalization of the E3 ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), in glioblastoma uncouples negative feedback between the pleckstrin homology domain leucine-rich repeat protein phosphatase 1 (PHLPP1) and Akt. J Biol Chem 2011 Jun [PMID: 21454620]
Liu J, Stevens PD, Gao T et al. mTOR-dependent regulation of PHLPP expression controls the rapamycin sensitivity in cancer cells. J Biol Chem 2011 Feb [PMID: 21177869]
Mistafa O, Ghalali A, Kadekar S et al. Purinergic receptor-mediated rapid depletion of nuclear phosphorylated Akt depends on pleckstrin homology domain leucine-rich repeat phosphatase, calcineurin, protein phosphatase 2A, and PTEN phosphatases. J Biol Chem 2010 Sep [PMID: 20605778]
Cleveland-Donovan K, Maile LA, Tsiaras WG et al. IGF-I activation of the AKT pathway is impaired in visceral but not subcutaneous preadipocytes from obese subjects. Endocrinology 2010 Aug [PMID: 20555032]
Brognard J, Niederst M, Reyes G et al. Common polymorphism in the phosphatase PHLPP2 results in reduced regulation of Akt and protein kinase C. J Biol Chem 2009 May [PMID: 19324870]
Gao T, Brognard J, Newton AC et al. The phosphatase PHLPP controls the cellular levels of protein kinase C. J Biol Chem 2008 Mar [PMID: 18162466]
Brognard J, Sierecki E, Gao T et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol Cell 2007 Mar [PMID: 17386267]

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PHLPP1
Entrez
Uniprot