PHAPIII Antibody

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Western Blot: PHAP III Antibody [NB100-94274] - Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with anti-PHAP III at 1 ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-94274
    • Availability
      Product Discontinued

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PHAPIII Antibody Summary

Immunogen
Rabbit anti-PHAP III polyclonal antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III (1).
Specificity
PHAP III
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANP32A
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Application Notes
This antibody can be used for detection of PHAP III by Western blot at 0.5 to 2 ug/ml. A549 or HepG2 cell lysate can be used as a positive control and a band at approximately 35 kDa can be detected. It is human, mouse, and rat reactive and has no cross-reaction to PHAP I and PHAP I2a.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
IgG purified

Alternate Names for PHAPIII Antibody

  • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
  • ANP32A
  • C15orf1
  • HPPCn
  • I1PP2A
  • LANP
  • MAPM
  • PHAP1
  • PHAPI
  • PP32

Background

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ANP32A
Entrez