PFKP Antibody (1D6)

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Western Blot: PFKP Antibody (1D6) [NBP2-01539] Analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PFKP Antibody (1D6) [NBP2-01539] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
Flow Cytometry: PFKP Antibody (1D6) [NBP2-01539] - Analysis of Jurkat cells, using anti-PFKP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PFKP Antibody (1D6) [NBP2-01539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFKP mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PFKP Antibody (1D6) Summary

Immunogen
Full length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PFKP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PFKP Antibody (1D6)

  • 6-phosphofructokinase type C
  • 6-phosphofructokinase, platelet type
  • EC 2.7.1
  • EC 2.7.1.11
  • PFK-CFLJ40226
  • PFKFplatelet type
  • Phosphofructokinase 1
  • phosphofructokinase, platelet
  • phosphohexokinase

Background

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PFKP
Entrez