PFKFB1 Antibody [HRP]

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Product Details

Summary
Product Discontinued
View other related PFKFB1 Primary Antibodies

Order Details


    • Catalog Number
      NB120-20563
    • Availability
      Product Discontinued

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PFKFB1 Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Fructose-6-Phosphate Kinase [Rabbit Muscle]
Localization
Cytoplasmic
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Gene
PFKFB1
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000
Readout System

Reactivity Notes

Rabbit

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
Gentamicin Sulfate
Purity
Ion exchange chromatography

Alternate Names for PFKFB1 Antibody [HRP]

  • 2-kinase:fructose-2,6-bisphosphatase
  • 6PF-2-K/Fru-2,6-P2ase liver isozyme
  • 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
  • F6PK
  • fructose-6-phosphate
  • MGC116715,6PF-2-K/Fru-2,6-P2ase 1
  • MGC116717
  • PFK/FBPase 1
  • PFRXHL2K

Background

Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PFKFB1