Peroxiredoxin 3 Recombinant Protein

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Western Blot: Peroxiredoxin 3 Protein [NBC1-27993] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1.0 mg/ml

Order Details

Peroxiredoxin 3 Recombinant Protein Summary

Description
An un-tagged recombinant protein corresponding to amino acids 63 to 256 of Human Peroxiredoxin 3

Source: E.coli

Amino Acid Sequence: MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ
Preparation
Method
E.coli
Details of Functionality
Specific activity: approximately 82-83 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute
Gene
PRDX3
Purity
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Notes

The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 21.5 kDa (195aa), confirmed by MALDI-TOF.

Alternate Names for Peroxiredoxin 3 Recombinant Protein

  • Antioxidant protein 1MGC104387
  • AOP-1
  • AOP-1MGC24293
  • AOP1PRO1748
  • EC 1.11.1.15
  • HBC189
  • MER5
  • Peroxiredoxin 3
  • Peroxiredoxin III
  • peroxiredoxin-3
  • PRDX3
  • Protein MER5 homolog
  • prx-III
  • SP-22
  • thioredoxin-dependent peroxide reductase, mitochondrial

Background

Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. Recombinant human Peroxiredoxin 3 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PRDX3
Entrez
Uniprot

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