PERK Antibody

Images

 
Western Blot: PERK Antibody [NB100-92406] - analysis of PERK (R87) pAb at 1:500 dilutionLane1:L02 whole cell lysate(40ug)Lane2:A549 whole cell lysate(40ug)
Immunohistochemistry-Paraffin: PERK Antibody [NB100-92406] - analyzes of PERK (R87) pAb in paraffin-embedded human esophagus carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the ...read more
Western Blot: PERK Antibody [NB100-92406] - Western blot (WB) analysis of PERK (R87) antibody in extracts from HeLa cells.
Western Blot: PERK Antibody [NB100-92406] Extracts from HeLa cells.
Immunohistochemistry-Paraffin: PERK Antibody [NB100-92406] - analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the ...read more
Immunohistochemistry-Paraffin: PERK Antibody [NB100-92406] - analyzes of PERK (R87) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the ...read more

Product Details

Summary
Product Discontinued
View other related PERK Primary Antibodies

Order Details


    • Catalog Number
      NB100-92406
    • Availability
      Product Discontinued

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PERK Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2AK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PERK (R87) pAb in extracts from HeLa cells.
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-92406.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PERK Antibody

  • DKFZp781H1925
  • EC 2.7.11.1
  • EIF2AK3
  • eukaryotic translation initiation factor 2 alpha kinase 3
  • eukaryotic translation initiation factor 2-alpha kinase 3
  • hsPEK
  • PEK
  • PEKPERKPancreatic eIF2-alpha kinase
  • PERK
  • PRKR-like endoplasmic reticulum kinase
  • WRS

Background

An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the alpha subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2alpha on serine-51 results in inhibition of translation. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2alpha, thus inhibiting the translation of mRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PERK Antibody (NB100-92406)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PERK Antibody (NB100-92406) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PERK Products

Bioinformatics Tool for PERK Antibody (NB100-92406)

Discover related pathways, diseases and genes to PERK Antibody (NB100-92406). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PERK Antibody (NB100-92406)

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Pathways for PERK Antibody (NB100-92406)

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PTMs for PERK Antibody (NB100-92406)

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Research Areas for PERK Antibody (NB100-92406)

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Blogs on PERK.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

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Bioinformatics

Gene Symbol EIF2AK3
Entrez