Perforin Antibody (dG9) [FITC]

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Flow Cytometry: Perforin Antibody (dG9) [FITC] [NB100-77864] - Whole blood lymphocytes stained intracellularly with dG9 FITC

Product Details

Summary
Product Discontinued
View other related Perforin Primary Antibodies

Order Details


    • Catalog Number
      NB100-77864
    • Availability
      Product Discontinued

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Perforin Antibody (dG9) [FITC] Summary

Immunogen
Purified granules from the human lymphoma cell line YT.
Specificity
Perforin (dG9)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per million cells in 100 microliter or 100 microliter of whole blood. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) and 0.2% (w/v) BSA
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Perforin Antibody (dG9) [FITC]

  • Cytolysin
  • FLH2
  • HPLH2
  • HPLH2lymphocyte pore forming protein
  • Lymphocyte pore-forming protein
  • MGC65093
  • P1PFN1
  • perforin 1 (pore forming protein)
  • perforin-1
  • PFPcytolysin

Background

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. The dG9 antibody recognizes human and bovine perforin and has been shown to be useful for flow cytometry, immunoprecipitation, immunohistochemistry, and immunofluorescence staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Perforin Antibody (NB100-77864) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Perforin Antibody (NB100-77864) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Perforin Products

Bioinformatics Tool for Perforin Antibody (NB100-77864)

Discover related pathways, diseases and genes to Perforin Antibody (NB100-77864). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Perforin Antibody (NB100-77864)

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Pathways for Perforin Antibody (NB100-77864)

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PTMs for Perforin Antibody (NB100-77864)

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Research Areas for Perforin Antibody (NB100-77864)

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Blogs on Perforin.

Perforin a Protective Serial Killer
Secretory granule-mediated cell death is one of the the key mechanisms for elimination of virus-infected and transformed target cells by cytotoxic lymphocytes. Formation of the immunological synapse between an effector and a target cell leads to exocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Perforin Antibodies for Detecting Immune System Diseases
 Perforin is a calcium-dependent pore forming cytolytic protein. Perforin is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20nm in diameter on target membranes. Killer lymphocy...  Read full blog post.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol PRF1