PEDF R/PNPLA2/ATGL Antibody

Images

 
Western Blot: ATGL Antibody [NB110-41536] - Detection of ATGL in human adipose tissue lysate.
Immunocytochemistry/ Immunofluorescence: ATGL Antibody [NB110-41536] - ATGL antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PEDF R/PNPLA2/ATGL Antibody Summary

Immunogen
A synthetic peptide within the C-terminal region (residues 400-500) of human ATGL. [Accession# NP_005171]
Localization
Cytoplasm, Adiposomes
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PNPLA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
Application Notes
This ATGL antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In ICC/IF, cytoplasmic/adiposomal staining was observed in HeLa cells.
Positive Control
PEDF R/PNPLA2/ATGL Lysate (NBL1-14554)
Control Peptide
PEDF R/PNPLA2/ATGL Peptide (NB110-41536PEP)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PEDF R/PNPLA2/ATGL Antibody

  • Adipose triglyceride lipase
  • ATGL
  • ATGL1110001C14Rik
  • Calcium-independent phospholipase A2
  • Desnutrin
  • EC 3.1.1.3
  • FP17548
  • IPLA2-zeta
  • patatin-like phospholipase domain containing 2
  • patatin-like phospholipase domain-containing protein 2
  • patatin-like phospholipase domain-containing protein 2-like
  • PEDF R
  • PEDFR
  • PEDF-R
  • Pigment epithelium-derived factor
  • PNPLA2
  • Transport-secretion protein 2
  • transport-secretion protein 2.2
  • triglyceride hydrolase
  • TTS2
  • TTS2.2
  • TTS-2.2
  • TTS2DKFZp667M109

Background

ATGL (adipose triglyceride lipase) is the rate-limiting lipolytic enzyme which catalyzes initial step of triglyceride (TAG) hydrolysis in adipocytes as well as non-adipocyte lipid droplets. Among adipose lipases (ATGL, hormone-sensitive lipase/HSL and monoacylglycerol lipase), HSL is a diacylglycerol lipase, whereas ATGL prefers TAG substrates. CGI-58 coactivates ATGL's reactions and G0/G1 switch gene 2 exerts supressive effects on ATGL. Localized in lipid droplet and cell membrane as single-pass type II membrane protein, ATGL is mainly expressed in adipose tissue, however, has also been reported in cardiac/skeletal muscles, gastrointestinal tract, normal retina and retinoblastoma. ATGL is inhibited by BEL and is activated by ABHD5 as well as SERPINF1, and is induced during differentiation of primary preadipocytes to adipocytes with expression increasing from fetal to adult in RPE cells. Besides TAG hydrolysis, ATGL has acylglycerol transacylase activity also and can act coordinately with LIPE/HLS within the lipolytic cascade, regulate size of adiposome and implicate in adiposomes degradation. ATGL implicates in energy homeostasis/response to starvation by enhancing TAGs hydrolysis and providing free fatty acids to be oxidized in situations of energy depletion. ATGL genetic variations have been linked with risk of NIDDM and neutral lipid storage disease with myopathy (NLSDM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-41536AF405
Alexa Fluor 488 Labeled NB110-41536AF488
Alexa Fluor 647 Labeled NB110-41536AF647
Alexa Fluor 700 Labeled NB110-41536AF700
Biotin Labeled NB110-41536B
DyLight 405 Labeled NB110-41536V
DyLight 488 Labeled NB110-41536G
DyLight 550 Labeled NB110-41536R
DyLight 650 Labeled NB110-41536C
FITC Labeled NB110-41536F
HRP Labeled NB110-41536H

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Bioinformatics

Gene Symbol PNPLA2
Entrez