PEDF R/PNPLA2/ATGL Antibody (2G2)

Images

 
Western Blot: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - The extracts of mouse kidney (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ATGL (1:1000). Proteins were visualized ...read more
Immunohistochemistry-Paraffin: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - Human ATGL (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clone
2G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PEDF R/PNPLA2/ATGL Antibody (2G2) Summary

Immunogen
Recombinant human ATGL (30-504aa) purified from E. coli
Specificity
Anti-human ATGL mAb, clone 2G2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATGL protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PNPLA2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:25-1:50
  • Immunohistochemistry-Paraffin 1:25-1:50
Application Notes
For IHC: Use 0.1M sodium citrate buffer for antigen retrieval and Diaminobenzidine (DAB) for detection. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25054327)
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-25852 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PEDF R/PNPLA2/ATGL Antibody (2G2)

  • Adipose triglyceride lipase
  • ATGL
  • ATGL1110001C14Rik
  • Calcium-independent phospholipase A2
  • Desnutrin
  • EC 3.1.1.3
  • FP17548
  • IPLA2-zeta
  • patatin-like phospholipase domain containing 2
  • patatin-like phospholipase domain-containing protein 2
  • patatin-like phospholipase domain-containing protein 2-like
  • PEDF R
  • PEDFR
  • PEDF-R
  • Pigment epithelium-derived factor
  • PNPLA2
  • Transport-secretion protein 2
  • transport-secretion protein 2.2
  • triglyceride hydrolase
  • TTS2
  • TTS2.2
  • TTS-2.2
  • TTS2DKFZp667M109

Background

Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PEDF R/PNPLA2/ATGL Antibody (NBP1-25852)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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(1)
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Bioinformatics

Gene Symbol PNPLA2
Entrez
Uniprot