Western Blot: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - The Tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were ...read more
Immunocytochemistry/ Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody ...read more
Flow Cytometry: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - Analysis of ATGL in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 ...read more
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25054327)
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-25852 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 10% Glycerol
0.02% Sodium Azide
Protein G purified
Alternate Names for PEDF R/PNPLA2/ATGL Antibody (2G2)
Adipose triglyceride lipase
Calcium-independent phospholipase A2
patatin-like phospholipase domain containing 2
patatin-like phospholipase domain-containing protein 2
patatin-like phospholipase domain-containing protein 2-like
Pigment epithelium-derived factor
Transport-secretion protein 2
transport-secretion protein 2.2
Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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