Western Blot: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - The extracts of mouse kidney (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ATGL (1:1000). Proteins were visualized ...read more
Immunohistochemistry-Paraffin: PEDF R/PNPLA2/ATGL Antibody (2G2) [NBP1-25852] - Human ATGL (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 10% Glycerol
0.02% Sodium Azide
Protein G purified
Western Blot 1:1000-1:2000
For IHC: Use 0.1M sodium citrate buffer for antigen retrieval and Diaminobenzidine (DAB) for detection. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25054327) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-25852 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for PEDF R/PNPLA2/ATGL Antibody (2G2)
Adipose triglyceride lipase
Calcium-independent phospholipase A2
patatin-like phospholipase domain containing 2
patatin-like phospholipase domain-containing protein 2
patatin-like phospholipase domain-containing protein 2-like
Pigment epithelium-derived factor
Transport-secretion protein 2
transport-secretion protein 2.2
Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.