PEA-15 Antibody

Images

 
Western Blot: Mat1 Antibody [NB100-92352] - Western blot (WB) analysis of Mat1 (E123) antibody in extracts from HeLa cells.

Product Details

Summary
Product Discontinued
View other related PEA-15 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92352
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

PEA-15 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human MNAT1.
Clonality
Polyclonal
Host
Rabbit
Gene
PEA15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Mat1 (E123) pAb in extracts from HeLa cells.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PEA-15 Antibody

  • HMAT1
  • HMAT115kD
  • HUMMAT1H
  • MAT1H
  • PEA15
  • PEA-15
  • PED
  • PED15 kDa phosphoprotein enriched in astrocytes
  • phosphoprotein enriched in astrocytes 15
  • Phosphoprotein enriched in diabetes

Background

Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160. The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. This previously undescribed protein, now termed Mat1 p36, has been cloned as a protein that associates with the cyclin H/Cdk7 nuclear complex at all stages of the cell cycle. Cyclin H/Cdk7/Mat1 p36 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II. Mat1 p36 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk/cyclin complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00000902-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-581
Species: Hu
Applications: WB, IHC, IP, PLA
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF2396
Species: Hu
Applications: WB, Simple Western, IHC
AF6349
Species: Hu, Mu, Rt
Applications: WB
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP2-56841
Species: Hu
Applications: WB, ICC/IF
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-77020
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-30371
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00002968-B01P
Species: Hu
Applications: WB, ICC/IF
NBL1-11392
Species: Hu
NBP1-86286
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NB100-92352
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for PEA-15 Antibody (NB100-92352) (0)

There are no publications for PEA-15 Antibody (NB100-92352).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PEA-15 Antibody (NB100-92352) (0)

There are no reviews for PEA-15 Antibody (NB100-92352). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PEA-15 Antibody (NB100-92352) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PEA-15 Products

Bioinformatics Tool for PEA-15 Antibody (NB100-92352)

Discover related pathways, diseases and genes to PEA-15 Antibody (NB100-92352). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PEA-15 Antibody (NB100-92352)

Discover more about diseases related to PEA-15 Antibody (NB100-92352).
 

Pathways for PEA-15 Antibody (NB100-92352)

View related products by pathway.

PTMs for PEA-15 Antibody (NB100-92352)

Learn more about PTMs related to PEA-15 Antibody (NB100-92352).
 

Research Areas for PEA-15 Antibody (NB100-92352)

Find related products by research area.

Blogs on PEA-15

There are no specific blogs for PEA-15, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PEA-15 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PEA15
Entrez