PDZK1 Antibody (NBP2-19761)

Images

 
Western Blot: PDZK1 Antibody [NBP2-19761] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP2-19761] - Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PDZK1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PDZK1. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Membrane; Peripheral membrane protein (By similarity)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDZK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (88%), Rabbit (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDZK1 Antibody

  • CAP70
  • CFTR-associated protein of 70 kDa
  • CLAMP
  • Na(+)/H(+) exchanger regulatory factor 3
  • Na/Pi cotransporter C-terminal-associated protein 1
  • NaPi-Cap1
  • NHERF3
  • NHERF-3
  • NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3
  • PDZ domain containing 1
  • PDZ domain-containing protein 1
  • PDZ-containing kidney protein 1
  • PDZD1
  • PDZK1
  • Sodium-hydrogen exchanger regulatory factor 3

Background

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDZK1
Entrez
Uniprot