PDX-1/IPF1 Antibody (2A12)

Images

 
Western Blot: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of HELA cells transiently transfected by pCMV6-ENTRY PDX1.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of HT29 cells using anti-PDX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (2A12) [NBP1-47910] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PDX-1/IPF1 Antibody (2A12) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-283 of human Pdx1 (NP_000200).
Specificity
This antibody is specific for Homo sapiens pancreatic and duodenal homeobox 1 (PDX1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:20000
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDX-1/IPF1 Antibody (2A12)

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

Pdx1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. It is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDX1
Entrez
Uniprot