PDPK1 Antibody

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Western Blot: PDPK1 Antibody [NBP2-44132] - Detection of myc-tagged human PDK-1 at 60kDa in 10 Ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 C with the

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.3 mg/ml

Order Details

PDPK1 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDPK1 protein.
Predicted Species
Mouse (92%), Rat (92%), Canine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDPK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:100000
  • Immunohistochemistry 1:10-1:500
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kDa in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.

Reactivity Notes

Cross reactivity with PDPK1 from other sources has not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDPK1 Antibody

  • 3-phosphoinositide dependent protein kinase-1
  • EC 2.7.11
  • EC 2.7.11.1
  • hPDK1
  • MGC20087
  • MGC35290
  • PDK1PRO0461
  • PkB kinase like gene 1
  • PkB-like 1,3-phosphoinositide-dependent protein kinase 1
  • protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDPK1