PDK-1 Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

PDK-1 Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
Localization
Mitochondrion
Predicted Species
Mouse (100%), Rat (100%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This PDK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with mouse and rat based on 100% sequence homology. Immunogen sequence has 93% homology to chicken.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PDK-1 Antibody [DyLight 488]

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
  • EC 2.7.11
  • EC 2.7.11.2
  • mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
  • PDK1
  • PDK-1
  • PDPK1
  • PKB kinase
  • Pyruvate dehydrogenase kinase isoform 1
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK-1 Antibody (NB100-2383G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-2383B
DyLight 550 Labeled NB100-2383R
DyLight 650 Labeled NB100-2383C
HRP Labeled NB100-2383H

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Calculators

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Bioinformatics

Gene Symbol PDK1
Entrez