PDK-1 Antibody (2D6)

Images

 
Western Blot: PDK-1 Antibody (2D6) [NBP1-30430] - The extracts of rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody (2D6) [NBP1-30430] - Analysis in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDK1 antibody (1:100) with goat ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-30430
    • Availability
      Product Discontinued

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PDK-1 Antibody (2D6) Summary

Immunogen
Recombinant human PDK1 (29-436aa) purified from E. coli
Specificity
This antibody is specific for human PDK1. It is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDK1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PDK1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250-1:1000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PDK-1 Antibody (2D6)

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
  • EC 2.7.11
  • EC 2.7.11.2
  • mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
  • PDK1
  • PDK-1
  • PDPK1
  • PKB kinase
  • Pyruvate dehydrogenase kinase isoform 1
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1

Background

PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDK1
Entrez
Uniprot