PDI Antibody

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Western Blot: PDI Antibody [NB100-58967] - Western blot analysis of PDI using this antibody at 0.03 ug/ml in Human Pancreas lysate (35ug protein in RIPA buffer).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PDI Antibody Summary

Immunogen
Peptide with sequence C-NLETTKKYAPVD corresponding to internal region according to NP_006840.2.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDIA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 60 kDa band observed in human pancreas lysates (calculated MW of 58.2 kDa band according to NP_006840.2).
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Pancreas Lysate (NB820-59244)
Publications
Read Publication using NB100-58967.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDI Antibody

  • Pancreas-specific protein disulfide isomerase
  • pancreatic protein disulfide isomerase
  • PDA2
  • PDI
  • PDIp
  • PDIPEC 5.3.4.1
  • PDIR
  • protein disulfide isomerase A2
  • protein disulfide isomerase family A, member 2
  • protein disulfide isomerase, pancreatic
  • protein disulfide isomerase-associated 2
  • protein disulfide-isomerase A2
  • Rho GDP dissociation inhibitor gamma

Background

Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully folded state and therefore the enzyme acts to catalyze protein folding. Another major function of PDI relates to its activity as a chaperone, in that it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells and is required for HIV infection of lymphocytes and monoctyes. Some studies have shown it to be available for HIV infection on the surface of the cell clustered around the CD4 protein yet conflicting studies have shown that it is not available on the cell surface but instead is found in significant amounts in the blood plasma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDI Antibody (NB100-58967)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDI Antibody (NB100-58967) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PDI Antibody (NB100-58967)

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Blogs on PDI.

ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.

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Bioinformatics

Gene Symbol PDIA2
Entrez
Uniprot