PDGFA Antibody

Product Discontinued

PDGFA Antibody Summary

• Immunogen: A fusion recombinant protein derived from E Coli, containing 196aa- full length of human PDGF protein
• Specificity: This antibody recognizes ~23 kDa of human PDGF protein
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDGFA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.01-0.1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Immunoprecipitation 2-5 ug/ml
  • Western Blot 0.1-1 ug/ml
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactive in Human, Rat, and Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile TBS (pH 7.2) with antibody stabilizer
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDGFA Antibody

• PDGF-1
• PDGF1PDGF A-chain
• PDGF-A
• Platelet-derived growth factor A chain
• platelet-derived growth factor alpha chain
• platelet-derived growth factor alpha isoform 2 preproprotein
• platelet-derived growth factor alpha polypeptidePDGF subunit A
• platelet-derived growth factor subunit A

Background

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of homo/hetero dimeric proteins, PDGF-AA, -BB, -CC, -DD and -AB. The PDGF-AA, -BB and AB bind to alpha type receptor, while beta type receptors bind to PDGF-BB and PDGF-AB. Upon activation by PDGF, the receptors get dimerised and auphosphorylated in several sites on its receptors (Receptor tyrosine kinase) in cytosolic domain to trigger cell signaling through PI3K pathway. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFA Antibody (NBP1-72192)(1)

Publication using NBP1-72192

• Chen WV, Delrow J, Corrin PD, Frazier JP, Soriano P Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Nat Genet. 36(3):304-12. 2004-03-01 [PMID: 14981515]

Bioinformatics

• Gene Symbol: PDGFA
• Entrez:
  • 5154(Human)
  • 18590(Mouse)
  • 25266(Rat)
• Uniprot:
  • P04085()