PDGFA Antibody

Product Discontinued

PDGFA Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 121-160 of Human PDGF-A.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDGFA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of PDGF-A (K154) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of PDGF-A (K154) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDGFA Antibody

• PDGF-1
• PDGF1PDGF A-chain
• PDGF-A
• Platelet-derived growth factor A chain
• platelet-derived growth factor alpha chain
• platelet-derived growth factor alpha isoform 2 preproprotein
• platelet-derived growth factor alpha polypeptidePDGF subunit A
• platelet-derived growth factor subunit A

Background

PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF-AA, PDGF-AB and PDGF-BB. The three isoforms bind with different affinities to two receptor types, alpha and beta, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to alpha receptors with high affinity, whereas the B subunit can bind to both alpha and beta receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFA Antibody (NBP1-19781)(2)

Publications using NBP1-19781

• Yu Guanzhen, Zhou Aidong, Xue Jianfei et al. FoxM1 promotes breast tumorigenesis by activating PDGF-A and forming a positive feedback loop with the PDGF/AKT signaling pathway. Oncotarget 2015-01-01 [PMID: 25869208] (WB, WB, Human)
• Wehler TC, Hamdi S, Maderer A et al. Single-agent therapy with sorafenib or 5-FU is equally effective in human colorectal cancer xenograft-no benefit of combination therapy Int J Colorectal Dis 2012-09-15 [PMID: 22983756] (IF/IHC, Mouse, Human)

Bioinformatics

• Gene Symbol: PDGFA
• Entrez:
  • 5154(Human)
  • 18590(Mouse)
  • 25266(Rat)
• Uniprot:
  • P04085()