PDGF R alpha Antibody [Unconjugated]

Images

 
PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue ...read more
PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was ...read more
Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) ...read more
Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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PDGF R alpha Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Specificity
Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRA
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF1062 in the following applications:

Publications
Read Publications using
AF1062 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R alpha Antibody [Unconjugated]

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGF R alpha Antibody (AF1062)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 17 applications: ICC, IF, IHC, IHC - Frozen, IHC - Not specified, IHC - PFA fixed, IHC - Paraffin embedded, IHC Fluorescence, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-Fr, IHC-P, IP, In vivo, WB.


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ICC
(5)
IF
(1)
IHC
(7)
IHC - Frozen
(2)
IHC - Not specified
(2)
IHC - PFA fixed
(1)
IHC - Paraffin embedded
(2)
IHC Fluorescence
(1)
IHC Frozen
(2)
IHC Not Specified
(1)
IHC OCT-embedded
(3)
IHC Paraffin-embedded
(3)
IHC-Fr
(1)
IHC-P
(2)
IP
(1)
In vivo
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(36)
Rat
(1)
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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using AF1062 Applications Species
K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams Pulmonary pericytes regulate lung morphogenesis Nat Commun, 2018;9(1):2448. 2018 [PMID: 29934496] (IHC-P,, Mouse) IHC-P Mouse
M Maden, JO Brant, A Rubiano, AGW Sandoval, C Simmons, R Mitchell, H Collin-Hoo, J Jacobson, S Omairi, K Patel Perfect chronic skeletal muscle regeneration in adult spiny mice, Acomys cahirinus Sci Rep, 2018;8(1):8920. 2018 [PMID: 29892004] (IHC,, Mouse) IHC Mouse
SN Kim, S Akindehin, HJ Kwon, YH Son, A Saha, YS Jung, JK Seong, KM Lim, JH Sung, KR Maddipati, YH Lee Anti-inflammatory role of 15-lipoxygenase contributes to the maintenance of skin integrity in mice Sci Rep, 2018;8(1):8856. 2018 [PMID: 29891910] (IHC-P,, Mouse) IHC-P Mouse
S Tanabe, T Yamashita B-1a lymphocytes promote oligodendrogenesis during brain development Nat. Neurosci., 2018;0(0):. 2018 [PMID: 29507409] (ICC,, Mouse) ICC Mouse
W Yu, WG Hill, SC Robson, ML Zeidel Role of P2XReceptor in Mouse Voiding Function Sci Rep, 2018;8(1):1838. 2018 [PMID: 29382907] (IHC-Fr,, Mouse) IHC-Fr Mouse
S Mitew, I Gobius, LR Fenlon, SJ McDougall, D Hawkes, YL Xing, H Bujalka, AL Gundlach, LJ Richards, TJ Kilpatrick, TD Merson, B Emery Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner Nat Commun, 2018;9(1):306. 2018 [PMID: 29358753] (IHC,, Mouse) IHC Mouse
Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination PLoS ONE, 2017;12(12):e0189380. 2017 [PMID: 29253893] (IHC - Frozen,, Mouse) IHC - Frozen Mouse
H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice Cell. Physiol. Biochem., 2017;43(1):158-171. 2017 [PMID: 28848093] (IHC,, Mouse) IHC Mouse
SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia PLoS ONE, 2017;12(8):e0182265. 2017 [PMID: 28806761] (IHC Frozen,, Mouse) IHC Frozen Mouse
H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries Dis Model Mech, 2017;0(0):. 2017 [PMID: 28714851] (IHC,, Mouse) IHC Mouse
Show All 38 Publications.

Reviews for PDGF R alpha Antibody (AF1062) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1062:
Filter by Applications
ICC
(1)
IHC-Fr
(1)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry PDGF R alpha AF1062
Enlarge
reviewed by:
Xing Fu
ICC Mouse 08/12/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse skeletal muscle primary cells,mouse skeletal muscle-derived cells,mouse skeletal muscle cells
SpeciesMouse
LotHMQ0217111
Immunohistochemistry-Frozen PDGF R alpha AF1062
Enlarge
reviewed by:
Michelle Tallquist
IHC-Fr Mouse 01/07/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult Mouse Lung Tissue,Adult heart
SpeciesMouse
LotHMQ0215021

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Xing Fu
08/12/2018
Application: ICC
Species: Mouse

Michelle Tallquist
01/07/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PDGFRA
Entrez
Uniprot