PDGF R alpha Antibody (RM0004-3G28)

Images

 
Immunohistochemistry-Paraffin: PDGF Receptor alpha Antibody (RM0004-3G28) [NB110-61020] - PDGFR-a immunohistochemistry staining of paraffin sections of mouse pancreas from LPS exposed animals.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
RM0004-3G28
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

PDGF R alpha Antibody (RM0004-3G28) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse PDGF receptor-alpha extracellular domain.
Specificity
PDGF receptor alpha (RM0004-3G28). This antibody recognizes mouse PDGF receptor alpha in western blot. No cross reactivity to mouse PDGF receptor beta.
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
PDGFRA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400-1:800
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This antibody is useful in Western blot and immunohistochemistry. For immunohistochemsitry, it is critical to use a mouse IgG preabsorbed secondary antibody.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGF R alpha Antibody (RM0004-3G28)

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDGFRA
Entrez
Uniprot