PDGF R alpha Antibody (APA5)

Images

 
Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Analysis using the Biotin conjugate of NBP1-43350. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by ...read more
Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr
Clone
APA5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

PDGF R alpha Antibody (APA5) Summary

Immunogen
The immunogen for this antibody was CD140a.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
PDGFRA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for PDGF R alpha Antibody (APA5)

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43350AF405
Alexa Fluor 488 Labeled NBP1-43350AF488
Alexa Fluor 647 Labeled NBP1-43350AF647
Alexa Fluor 700 Labeled NBP1-43350AF700
Allophycocyanin Labeled NBP1-43350APC
Biotin Labeled NBP1-43581
DyLight 350 Labeled NBP1-43350UV
DyLight 405 Labeled NBP1-43350V
DyLight 488 Labeled NBP1-43350G
DyLight 680 Labeled NBP1-43350FR
DyLight 755 Labeled NBP1-43350IR
PE Labeled NBP1-43350PE
PerCP Labeled NBP1-43350PCP

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Bioinformatics

Gene Symbol PDGFRA
Entrez
Uniprot