Human PDGF-CC Recombinant Protein

Product Discontinued

Human PDGF-CC Recombinant Protein Summary

• Description: A disulfide-linked homodimeric protein with a C-terminal poly-His tag corresponding to amino acids Val235 - Gly345 of PDGF-CC.
  
  

  Source: E. coli

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: The ED50, as determined by a cell proliferation assay using NR6R-3T3 cells, is less than 350 ng/mL.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDGFC
• Endotoxin Note: Less than 1 EU/mg of the rHuPDGF-CC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with 4 mM HCl to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human PDGF-CC Recombinant Protein

• PDGFCC
• PDGF-CC

Background

PDGF-CC is a 32 kDa homodimeric growth factor that is secreted by platelets, vascular and visceral smooth muscle cells, renal mesangial cells, macrophages, and retinal pigment epithelium. PDGF-CC is mitogenic toward retinal pigment cells and fibroblasts. It also is a chemoattractant for macrophages and endothelial cell precursors, and induces MMP expression by phagocytes. PDGF-CC binds and signals through homodimers of PDGF Ra and heterodimeric PDGF Ra/b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDGFC