PDF Antibody (3F3)

Images

 
Western Blot: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: PDF Antibody (3F3) [NBP2-00814] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.
Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody, and then analyzed by ...read more
Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Hela cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: PDF Antibody (3F3) [NBP2-00814] - Analysis of Jurkat cells, using anti-PDF antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
3F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PDF Antibody (3F3) Summary

Immunogen
Full length human recombinant protein of human PDF (NP_071736) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDF Antibody (3F3)

  • EC 3.5.1.88
  • GDF15
  • MIC1
  • PDF1A
  • peptide deformylase (mitochondrial)
  • peptide deformylase, mitochondrial
  • peptide deformylase-like protein
  • PLAB
  • Polypeptide deformylase

Background

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDF
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