PDE5A Antibody

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Product Details

Summary
Product Discontinued
View other related PDE5A Primary Antibodies

Order Details


    • Catalog Number
      NB110-40466
    • Availability
      Product Discontinued

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PDE5A Antibody Summary

Immunogen
Synthetic peptide - KLH conjugated near N-terminus of human
Epitope
near N-terminus of human
Specificity
This is specific to the human PDE5A protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PDE5A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 500 ng/ml

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE5A Antibody

  • CGB-PDE
  • cGMP-binding cGMP-specific 3'-5'-cyclic nucleotide phosphodiesterase
  • cGMP-binding cGMP-specific phosphodiesterase
  • cGMP-specific 3'-5'-cyclic phosphodiesterase
  • cGMP-specific phosphodiesterase type 5A
  • CN5A
  • EC 3.1.4
  • EC 3.1.4.35
  • PDE5
  • phosphodiesterase 5A, cGMP-specific
  • phosphodiesterase isozyme 5

Background

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A (PDE5A). The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB PDEs). The cGB PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP specific phosphodiesterase type 5A (PDE5A) family is comprised of 2 genes (PDE5A and PDE5B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP dependent kinase phosphorylation. PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP kinases. There is growing evidence that supports important roles for PDE5A in both the vasculature and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDE5A
Entrez