PDE4B Antibody (1D12)

Images

 
Western Blot: PDE4B Antibody (1D12) [NBP2-01171] Analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: PDE4B Antibody (1D12) [NBP2-01171] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4B mouse monoclonal antibody.
Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Jurkat cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PDE4B Antibody (1D12) [NBP2-01171] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4B mouse monoclonal antibody.
Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Hela cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PDE4B Antibody (1D12) Summary

Immunogen
Full length human recombinant protein of human PDE4B(NP_002591) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PDE4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDE4B Antibody (1D12)

  • cAMP-specific (phosphodiesterase E4 dunce homolog
  • cAMP-specific 3'-5'-cyclic phosphodiesterase 4B
  • cAMP-specific phosphodiesterase-4 B isoform
  • DKFZp686F2182
  • Drosophila)
  • dunce-like phosphodiesterase E4
  • EC 3.1.4
  • EC 3.1.4.17
  • MGC126529
  • PDE32
  • PDEIVB
  • phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E4)
  • phosphodiesterase 4B, cAMP-specific (dunce
  • phosphodiesterase 4B, cAMP-specific
  • phosphodiesterase E4 dunce homolog

Background

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PDE4B
Entrez