PD-L1/B7-H1 Antibody

Images

 
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. The HRP-conjugated ...read more
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and ...read more
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:600 and with DDDDK tag antibody (NBP2-43574) ...read more
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated ...read more
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used ...read more
Immunocytochemistry/ Immunofluorescence: PD-L1/B7-H1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells. Green: PD-L1 protein stained by PD-L1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. ...read more
Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 ...read more
Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit ...read more
Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
33 mg/ml
 

Independent Antibodies

 

Biological Strategies

 

Genetic Strategies

   

Order Details

PD-L1/B7-H1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.
Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Endomembrane system
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD274
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
0.24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
33 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PD-L1/B7-H1 Antibody

  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PDL1
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1

Background

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1086
Species: Hu
Applications: WB, Flow, IHC, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-76770
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
MAB7158
Species: Hu, Mu
Applications: WB, IHC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
MAB342
Species: Hu
Applications: WB, AgAct
NB100-39002
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF165
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-12499
Species: Hu
Applications: IHC, IHC-P
NBP2-15791
Species: Hu
Applications: WB, ICC/IF, IHC-P

Publications for PD-L1/B7-H1 Antibody (NBP2-15791) (0)

There are no publications for PD-L1/B7-H1 Antibody (NBP2-15791).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PD-L1/B7-H1 Antibody (NBP2-15791) (0)

There are no reviews for PD-L1/B7-H1 Antibody (NBP2-15791). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PD-L1/B7-H1 Antibody (NBP2-15791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PD-L1/B7-H1 Products

Bioinformatics Tool for PD-L1/B7-H1 Antibody (NBP2-15791)

Discover related pathways, diseases and genes to PD-L1/B7-H1 Antibody (NBP2-15791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PD-L1/B7-H1 Antibody (NBP2-15791)

Discover more about diseases related to PD-L1/B7-H1 Antibody (NBP2-15791).
 

Pathways for PD-L1/B7-H1 Antibody (NBP2-15791)

View related products by pathway.

PTMs for PD-L1/B7-H1 Antibody (NBP2-15791)

Learn more about PTMs related to PD-L1/B7-H1 Antibody (NBP2-15791).
 

Research Areas for PD-L1/B7-H1 Antibody (NBP2-15791)

Find related products by research area.

Blogs on PD-L1/B7-H1

There are no specific blogs for PD-L1/B7-H1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PD-L1/B7-H1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CD274
Entrez
Uniprot