PD-L1/B7-H1 Antibody

Images

 
Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NB300-903] - B7-H1/PD-L1/CD274 Antibody [NB300-903] - B7-H1/PD-L1/CD274 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with ...read more
Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NB300-903] - B7-H1/PD-L1/CD274 Antibody [NB300-903] - (2ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, ...read more
Western Blot: B7-H1/PD-L1/CD274 Antibody [NB300-903] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PD-L1/B7-H1 Antibody Summary

Immunogen
Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1; NP_001254635.1
Specificity
This antibody is expected to recognize reported isoforms a and b (NP_054862.1; NP_001254635.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD274
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx 40kDa band observed in Human Heart lysates (calculated MW of 33.3kDa according to NP_054862.1). The observed molecular weight corresponds to the glycosylated forms.
IHC-P: Human placenta shows membranous staining of cytotrophoblasts. Data obtained from a previous batch (from a different goat).
Publications
Read Publication using NB300-903.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PD-L1/B7-H1 Antibody

  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PDL1
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-L1/B7-H1 Antibody (NB300-903)(1)

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Bioinformatics Tool for PD-L1/B7-H1 Antibody (NB300-903)

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Bioinformatics

Gene Symbol CD274
Entrez
Uniprot