PD-L1/B7-H1 Antibody (MIH5)

Images

 
Immunocytochemistry/ Immunofluorescence: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in mouse ...read more
Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with ...read more
Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human colon cancer cells using ...read more
Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a ...read more
Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used ...read more
Flow Cytometry: B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total ...read more
Flow Cytometry: B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr
Clone
MIH5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

PD-L1/B7-H1 Antibody (MIH5) Summary

Immunogen
The immunogen for this antibody was B7H1.
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
CD274
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-43262 in the following applications:

Publications
Read Publications using NBP1-43262.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for PD-L1/B7-H1 Antibody (MIH5)

  • anti-PD-L1
  • B7-H
  • B7H1
  • B7-H1
  • B7H1PDCD1L1
  • CD274 antigenMGC142294
  • CD274 molecule
  • CD274
  • PDCD1L1
  • PDCD1LG1
  • PDCD1LG1MGC142296
  • PD-L1 blocking
  • PD-L1 ihc
  • PD-L1 immunohistochemistry
  • PD-L1 western blot
  • PDL1
  • PD-L1
  • PD-L1B7 homolog 1
  • PDL1PDCD1 ligand 1
  • programmed cell death 1 ligand 1
  • Programmed death ligand 1

Background

The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-L1/B7-H1 Antibody (NBP1-43262)(4)


Showing Publications 1 - 4 of 4.
Publications using NBP1-43262 Applications Species
Sheng H, Wang Y, Jin Y et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1 Cell Res 2008 Aug [PMID: 18607390]

Details:
This citation used the Biotin version of this antibody.
Tsushima F, Iwai H, Otsuki N et al. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses Eur J Immunol 2003 Oct [PMID: 14515261]

Details:
This citation used the Biotin version of this antibody.
Sheng H, Wang Y, Jin Y et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1 Cell Res

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-43506).
Tsushima F, Iwai H, Otsuki N et al. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses Eur J Immunol

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-43506).

Review for PD-L1/B7-H1 Antibody (NBP1-43262) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-43262:
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ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Human 06/21/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PD-L1/B7-H1 Antibody (NBP1-43262). (Showing 1 - 2 of 2 FAQs).

  1. Can you recommend a mouse tissue positive control for this antibody hen used in IHC labelling?
    • CD274 in mouse is highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin. (UniProt Q9EP73) I would recommend trying heart, thymus, skeletal muscle, or lung for IHC. Here is a publication that used this clone with IHC staining of mouse tissues for your reference as well: Tsushima et al. Eur J Immunol. 2003 Oct;33(10):2773-82
  2. On your website, it states that clone MIH5 binds to both human and mouse CD274. However, other companies state this clone only binds to mouse. What type of validation do you have that this binds to human CD274?
    • This antibody was tested on human primary colon cells and the cancer cells including HT29 cell line by one of our customer, and you can see the related data on the datasheet of this product. Datasheet

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43262AF405
Alexa Fluor 488 Labeled NBP1-43262AF488
Alexa Fluor 647 Labeled NBP1-43262AF647
Alexa Fluor 700 Labeled NBP1-43262AF700
Allophycocyanin Labeled NBP1-43262APC
Biotin Labeled NBP1-43506
DyLight 350 Labeled NBP1-43262UV
DyLight 405 Labeled NBP1-43262V
DyLight 488 Labeled NBP1-43262G
DyLight 680 Labeled NBP1-43262FR
DyLight 755 Labeled NBP1-43262IR
PE Labeled NBP1-43262PE
PerCP Labeled NBP1-43262PCP

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Recent Reviews

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Anonymous
06/21/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CD274
Entrez