PD-1 Antibody (RMP1-30) [FITC]

Images

 
Flow Cytometry: PD-1 Antibody (RMP1-30) [FITC] [NBP2-00293] - Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated C57Bl/6 splenocytes with 0.25 ug of Rat IgG2b K Isotype Control ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
RMP1-30
Clonality
Monoclonal
Host
Rat
Concentration
0.5 mg/ml

Order Details

PD-1 Antibody (RMP1-30) [FITC] Summary

Immunogen
Mouse PD-1 transfected BHK cells
Specificity
PD-1 (RMP1-30)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
PDCD1
Purity
Protein A or G purified
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
FITC
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
Flow Cytometry and Western Blot For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume.

Alternate Names for PD-1 Antibody (RMP1-30) [FITC]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDCD1
Entrez
Uniprot

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