PCNA Antibody [Biotin]


Western Blot: PCNA Antibody [Biotin] [NBP1-71795] - Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 seconds.

Product Details

Reactivity Hu, Mu, Alligator, Bovine, Canine, Chicken, Equine, Guinea Pig, Primate, Porcine, Rabbit, Rat, Turkey, Rhesus MacaqueSpecies Glossary
Applications WB, Flow, IP
LYOPH mg/ml

Order Details

PCNA Antibody [Biotin] Summary

The immunogen this antibody was made to, maps to a region between residues 75-125 of human proliferating cell nuclear antigen using the SwissProt entry P12004 (GeneID 5111).
Proliferation Marker
Predicted Species
Rat (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Turkey (100%), Canine (100%), Bovine (100%), Primate (100%), Guinea Pig (100%), Equine (100%), Chicken (100%), Alligator (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
PBS and 0.2% BSA
0.09% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:1000-1:10000
  • Flow Cytometry 3 ug per 1 million cells in a 150 mcl reaction
  • Immunoprecipitation 2 - 5 ug/mg lysate
Readout System

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, American alligator, White-tufted-ear marmoset, Crab-eating macaque and American mink.

Alternate Names for PCNA Antibody [Biotin]

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on PCNA.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Product PDFs


Gene Symbol PCNA

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